Periostin ELISA 试剂盒
Quick Overview for Periostin ELISA 试剂盒 (ABIN2111982)
抗原
See all Periostin (POSTN) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 78 pg/mL
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品牌
- BioAssay™
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Analytical Method
- Quantitative
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灵敏度
- 15 pg/mL
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组件
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Human periostin Standard, lyophilized, 1x1ug
Detection Antibody, 1x20 µL
HRP Labeled Streptavidin, lyophilized, 1x2ug
Wash Buffer 10X, 2x30 mL
ELISA Buffer 10X, 2x30 mL
TMB Substrate Solution, 1x10 mL
Stop Solution, 1x10 mL
Plate coated with periostin antibody, 6x16 well strips
Plate covers, 2x plastic film
Silica gel, 2x minibags
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Periostin (POSTN) ELISA Kit
POSTN 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 小鼠 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Periostin (POSTN) ELISA KitPOSTN 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Periostin (POSTN) ELISA KitPOSTN 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Periostin (POSTN) ELISA KitPOSTN 适用: 大鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma, Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 人 AA 22-836 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 小鼠 AA 24-836 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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板类型
- Pre-coated
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实验流程
- This BioAssay-is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human periostin in cell culture supernatants, serum and plasma. A monoclonal antibody specific for periostin has been precoated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, periostin is recognized by the addition of a biotinylated monoclonal antibody specific for periostin (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3-,5,5-- tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of periostin in the samples.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- Periostin (POSTN)
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别名
- Periostin
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背景
- Periostin was originally isolated as an osteoblast-specific factor that functions as a cell adhesion molecule for pre-osteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta1 (TGF-beta1) and bone morphogenetic protein 2 (BMP-2). Abnormal expression of periostin is also linked to angiogenesis and metastasis in epithelial tumors. Periostin up-regulation in cancers usually correlates with aggressiveness and/or poor survival.
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